Coding
VHH

Part:BBa_K4387989:Design

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-29)


Bivalent Anti-Tumour Necrosis Factor Nanobody (VHH#2B + VHH#12B)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence was converted into DNA and then codon optimized for E. coli with the help of the codon optimization tool provided by Integrated DNA Technologies (IDT). The same linker was used as the one described in the patent. [1]


Source

The amino acid sequence was directly taken from the patent mentioned in the description. [1]


References

  • [1] Silence, Karen, Lauwereys, Marc, De Haard, Hans, et al. "Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor", Int. Publication Number: WO 2004/041862 A2, 21 May 2004